Human Granulocyte Colony - Stimulating Factor Specifically Binds to Murine

Comparison of T7- and Lac-Based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Factor in Escherichia coli

Expression and Secretion of Human Granulocyte Macrophage-Colony Stimulating Factor Using Escherichia coli Enterotoxin I Signal Sequence

With the aim of the secretion of human granulocyte macrophage-colony stimulating factor (hGM-CSF) in Escherichia coli, hGM-CSF cDNA was fused in-frame next to the signal sequence of ST toxin (ST-I) of exteroxigenic E. coli, containing 53 or 19 amino acids of signal peptide. The fused STsig::hGM-CSF coding fragments were inserted into a T7-based expression plasmid. The recombinant plasmids were ...

The Expression of Human Granulocyte Macrophage Colony Stimulating Factor by Heat-Induction in Escherichia coli

A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...

Human Granulocyte Colony - Stimulating Factor Specifically Binds to Murine Myeloblastic NFS - 60

The specific binding of human granulocyte colony-stimulating factor (G-CSF) to its receptors on NFS-60 cells acts as a primer for cellular proliferation. There are approximately 400 binding sites per cell. with a binding constant of about 1 00 pmol/ 1. Before the proliferative response, the affinity constant of the membrane particulate fraction to labeled guanosine triphosphate-gamma-S ( ‘S-GTP...

Improvement in Development and Quality of 8 Cell Mouse Embryos in Presence of Granulocyte Macrophage-Colony Stimulating Factor

Purpose: Granulose Macrophage-Colony Stimulating Factor (GM-CSF) is a lympho- heamatopoietic actor, secreted in the reproductive system. Murine pre- implantation embryos express GM-CSF receptors. In his study, the capacity of eight cell mouse embryos was studied in the presence and absence of GM-CSF. Materials and Methods: Female NMRI mice were super ovulated using Pregnant Mare Serum gonadotr...